Fig. 2

Deletion of elovl1a and elovl1b genes in zebrafish and their effects on fatty acid compositions

(A) The targeting site for elovl1a and elovl1b gene knockout. The gray boxes were the 5′-untranslated and 3′-untranslated regions, and blue boxes the exons. The red boxes indicated the targeting sequences.

(B) Mutation confirmation, as shown by the results of the protein expression of Elovl1a and Elovl1b.

(C and D) The total SFA, MUFA, and PUFA contents in livers of 2-month-old WT, elovl1a^–/– (C) and elovl1b^–/– (D) zebrafish.

(E and F) Fatty acid compositions in livers of 2-month-old elovl1a^–/– (E) and elovl1b^–/– (F) zebrafish. The blue and orange colors in (E) and (F), respectively, meant significant decrease and increase in parameters of elovl1a^–/–/elovl1b^–/– zebrafish, compared with WT zebrafish (p < 0.05). Data were given as means ± SD of three or four biological replicates. The statistical analyses were conducted by t test. WT, wild-type zebrafish; elovl1, fatty acyl elongase 1; elovl1a^–/–, elovl1a knockout zebrafish; elovl1b^–/–, elovl1b knockout zebrafish; SFA, saturated fatty acid; MUFA, monounsaturated fatty acid; PUFA, polyunsaturated fatty acid; Gapdh, glyceraldehyde-3-phosphate dehydrogenase.