FIGURE

Figure 7

ID

ZDB-FIG-221214-296

Publication

Ahmad-Mansour et al., 2022 - Characterization of the Secreted Acid Phosphatase SapS Reveals a Novel Virulence Factor of Staphylococcus aureus That Contributes to Survival and Virulence in Mice

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Figure 7

Impact of SapS on the phagocytosis of S.aureus strain SA564 by polymorphonuclear leukocytes (PMNs). The cells of S. aureus were labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE) and mixed to lithium heparin-anticoagulated fresh human blood at a multiplicity of infection of 50 per PMN, and maintained at 37 �C and 1000 rpm. Using flow cytometry, as described in the Material and Methods section, the adhesion and ingestion of bacteria by PMNs was examined. The data show three biological replicates that were performed in triplicate. Data are presented as box and whisker plots showing the interquartile range (25?75%; box), median (horizontal line), and whiskers (bars; min/max). ns, not significant (Kruskal?Wallis test followed by Dunn?s post hoc test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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