Fig. 5

Analysis of the mobility pattern of YFP-GR in zebrafish embryos. Data obtained from three embryos from both the vehicle- (red) and dexamethasone-treated (blue) groups were pooled, and their averages with standard deviations are presented. (A) Cumulative probability P plot of squared displacement measurements r², obtained experimentally for a time lag of 25 ms. Experimental data are best fitted using a two-population model, yielding the size of the fast subpopulation and the MSD for each time lag. Number of MSD replicates per time lag in each of the group equaled to 30. (B) Fast subpopulation fraction size in the vehicle-and dexamethasone-treated groups, fitted to a linear model with a fixed slope. P(t = 25 ms): 0.72, P(t = 50 ms): 0.49, P(t = 75 ms): 0.69, P(t = 100 ms): 0.92, P(t = 125 ms): 0.81. The 95% confidence interval and the standard deviation are shown. (C) MSD values for the fast subpopulation for the vehicle- and dexamethasone-treated groups, fitted using a confined diffusion model. The 95% confidence interval and the standard deviation are shown. P(t = 25 ms): 0.11, P(t = 50 ms): 0.02, P(t = 75 ms): < 0.01, P(t = 100 ms): < 0.01, P(t = 125 ms): < 0.01. (D) MSD values for the slow subpopulation for the vehicle- and dexamethasone-treated groups, fitted using a confined diffusion model. P(t = 25 ms): 0.15, P(t = 50 ms): 0.83, P(t = 75 ms): 0.24, P(t = 100 ms): 0.27, P(t = 125 ms): 0.42. The 95% confidence interval and the standard deviation are shown. For (B, C, D), Shapiro-Wilk test was performed to check if data is normally distributed. Significance of the results was conducted using a mixed-effect model for comparison of means between two normally distributed groups. Significant differences are indicated by an asterisk (Significance levels: P > 0.05, non-significant; P < 0.05, *; P < 0.01, **). Pearson?s correlation coefficients R² of the model fitness are shown.