FIGURE

Fig. 3

ID

ZDB-FIG-221209-17

Publication

Wang et al., 2021 - Dissecting VEGF-induced acute versus chronic vascular hyperpermeability: Essential roles of dimethylarginine dimethylaminohydrolase-1

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Fig. 3

Figure 3. DDAH1 mediates acute and chronic VEGF-mediated signaling pathways in ECs

(A) HUVECs were infected with lentivirus expressing of control shRNA and DDAH1 shRNA, cultured in low-serum EMB for 4 h and then exposed to stimuli of acute (single stimulus of VEGF at 10 ng/mL) and chronic VEGF (three stimuli of VEGF at 10 ng/mL with 30 min intervals), respectively. Proteins were collected 10 min after VEGF stimulation and analyzed with western blotting (A).

(B) Control and DDAH1 knockdown HUVECs were labeled with DAF-FM (2.5 μM) and then exposed to acute and chronic VEGF stimulation, respectively. DAF-FM fluorescence was measured at Excitation/Emission of 495/515 nm. N numbers are presented in the scatter plot. Data is expressed as mean ± SD. ∗∗∗, p < 0.001.

(C) HUVECs were infected with retrovirus expressing of LacZ and DDAH1, cultured in low-serum EMB and then exposed to acute and chronic VEGF stimulation. Proteins were collected 10 min after VEGF stimulation and analyzed with western blotting.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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