FIGURE
Fig. 4
- ID
- ZDB-FIG-221122-13
- Publication
- Treffy et al., 2021 - Neuroblastoma differentiation in vivo excludes cranial tumors
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Fig. 4
(A–D) Lateral view schematics of zebrafish embryos at 14 hpi that were injected with membrane-localized mCherry-expressing SK-N-AS NB cells that either differentiated into neurons (red) or not (blue) during time-lapse imaging. (A) Empty vector (EV) or scrambled vector (n = 30 embryos), duplicated from Figure 2B for clarity; (B) EV + 5 μM RA on cultured cells for 24 h pre-injection (n = 29 embryos); (C) EV + 1 μM RA on host embryos post-injection through time-lapse (n = 30 embryos); (D) EV + 50 μM DEAB on host embryos 2 h pre-injection through time-lapse (n = 16 embryos). (E and F) Displacement vectors for untreated SK-N-AS cells (n = 30 embryos) (E) and RA-treated SK-N-AS cells (n = 29 embryos) (F). Red and blue vectors are for neuronal and non-neuronal cells, respectively. (G) Aggregates of displacements for untreated and RA-treated SK-N-AS cells divided into neuronal and non-neuronal categories. Arrows are scaled, and their orientations correspond to orientations in (E) and (F). Net anterior and dorsal displacements, as well as absolute displacements across all axes, are shown along with p values. Displacements are affected by embryo growth such that dorsal values are skewed positively despite most cells migrating ventrally; therefore, comparisons are made only relative to appropriate controls. (H) Scatter plot of maximal projection lengths and their segregation into aROI and pROI groups stratified by tested conditions. Control aROI and pROI columns are duplicated from Figure 2I for clarity and are composed of scrambled and empty vector data points. Horizontal bars denote mean values, with 95% confidence intervals indicated. n.s. p > 0.05; ∗p < 0.05; ∗∗∗p < 0.001. ivt, in vitro; ivv, in vivo. Scale bar (A–F), 100 μm. Scale bar (G), 50 μm. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 56(19), Treffy, R.W., Rajan, S.G., Jiang, X., Nacke, L.M., Malkana, U.A., Naiche, L.A., Bergey, D.E., Santana, D., Rajagopalan, V., Kitajewski, J.K., O'Bryan, J.P., Saxena, A., Neuroblastoma differentiation in vivo excludes cranial tumors, 2752-2764.e6, Copyright (2021) with permission from Elsevier. Full text @ Dev. Cell