Fig. 8
- ID
- ZDB-FIG-220926-8
- Publication
- Jussila et al., 2022 - Live imaging and conditional disruption of native PCP activity using endogenously tagged zebrafish sfGFP-Vangl2
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FoxJ1a-lineage specific degradation of sfGFP-Vangl2 results in ectopic scospondin accumulation and disrupted Reissner fiber formation.
a, b Representative maximum intensity Z-stack projections of confocal micrographs, acquired through dorsally-oriented whole mount brains that were dissected from 21dpf vangl2sfGFP/sfGFP;Tg(βactin2::loxP-mCherry-STOP-loxP-zGrad) control (a; n = 4) and vangl2sfGFP/sfGFP;Tg(βactin2::loxP-mCherry-STOP-loxP-zGrad);Tg(foxj1a::iCre) mutant (b; n = 9) brains, and immunostained for polyglutamylated tubulin (magenta) and scospondin (green). Inverted, higher magnification images of scospondin immunostaining in forebrain (c, d) and rhombencephalic (e, f) ventricles of control (c, e) and mutant (d, f) brains, represented by boxed areas in a, b. |
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Stage: | Days 21-29 |
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Stage: | Days 21-29 |