Fig. 5

Loss of zrsr2 leads to the differential retention of U12-type introns in RNA and protein processing genes. (A) Pie charts depicting the classifications for all differentially retained introns between zrsr2^hg129/hg129 and WT embryos into U12-type (blue) and U2-type (orange) introns (left) and the further classification of the retained U2-type introns (right) by whether they are adjacent to a known U12-type intron (green) or not adjacent but in a gene with a U12-type intron (purple), or in a gene with no known U12-type introns (grey). Some of the retained introns could not be classified into either category (yellow). (B) Plots of intron size distribution in the retained U12-type introns (left) and the non-retained U12-type introns (right). (C) The top 10 biological processes enriched in all genes (612) containing U12-type introns in the zebrafish genome according to IAOD. (D) The top 10 biological processes enriched in the set of U12-type intron containing genes (221) that have a differential IR between WT and zrsr2^hg129/hg129 embryos at 3 dpf, with enrichment visualized by −log10 (p-value). Five of the ten top pathways (written in red) are common in both gene sets (C,D).