Fig. 4

µ-CT analyses of cardiovascular morphology in alk5a^?/? adult zebrafish reveal OFT defects. (A, B) µ-CT scans showing a sagittal plane of the anterior region of WT and alk5a^?/? adult zebrafish. Boxed area is shown in A? and B?. Dashed lines outline cardiac compartments. (C?D??) 3D reconstructions of the cardiac compartments in WT (C?C??) and alk5a^?/? (D?D??) zebrafish, including the aorta (C?, D?) and OFT lumen (Lu; C??, D??). (C??, D??) 3D reconstructions of the aortic opening (white line) as seen from the OFT. (E?J) Quantification of morphological parameters for each cardiac compartment in WT (n = 10) and alk5a^?/? (n = 12) zebrafish. Plots show the values for each individual and the mean ± SD; P-values were determined by unpaired t-test (G?J) or Mann?Whitney test (E, F). The colour of each dot refers to the same zebrafish across all graphs and images. The dot adjacent to the number symbol (#) identifies the individual zebrafish mentioned in the text. A, atrium; Ao, aorta; OFT, outflow tract; V, ventricle. Scale bars: 1 mm (A, B), 500 µm (A?, B?), 300 µm (C, D?), 400 µm (C??D??).