FIGURE

Fig. 1

ID
ZDB-FIG-220923-78
Publication
Williams et al., 2022 - Assessment of endocytic traffic and Ocrl function in the developing zebrafish neuroepithelium
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Fig. 1

Imaging of endocytic tracer uptake and trafficking in the zebrafish neuroepithelium. (A,B) Schematic of tissue organisation within the developing zebrafish brain in 1 dpf embryos as viewed by confocal microscopy from coronal (A) and sagittal (B) perspectives. Dashed boxed regions indicate the regions imaged. Neuroepithelial cell membranes are labelled with transiently expressed membrane-GFP. The positions of the forebrain (‘F’), midbrain (‘M’) and hindbrain (‘H’) ventricle are indicated, with the needle showing the region injected with endocytic tracers. Dashed lines indicate apical and basolateral neuroepithelial tissue boundaries. (C) Hindbrain ventricle injection of TxR–dextran accumulates in EGFP–Rab7-labelled compartments at 15 min post injection and to a greater degree at 60 min post injection. (D) Hindbrain ventricle injection of DQ-BSA imaged in live embryos 1 h post injection shows accumulation of DQ-BSA in EGFP–Rab7-positive compartments but not in EGFP–Rab5-labelled compartments. Arrowheads point to instances of co-localisation between stably expressed EGFP–Rab5 or EGFP–Rab7 and the indicated tracer. Images are representative of a minimum of two independent experiments. Scale bars: 50 µm (A, left); 10 µm (B); 5 µm (A, right; C,D).

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Cell Sci.