FIGURE 5

Eomesa is a more potent inducer of endoderm, organiser and dorsal forerunner cell markers than other T-box factors. (A–C) WISH analysis of dorsal mesoderm marker noto(A) and dorsal forerunner cell markers sox32(B) and vgll4l(C) on overexpression of various wild type and mutant T-box factors. mRNAs injected at the 1 cell stage; WISH performed at stages as indicated. N = 2. Total numbers of embryos scored per condition are indicated. Representative images of expression patterns per gene per category are shown. Lateral views; dorsal to the right. Arrowheads indicate ectopic expression. Fisher’s Exact two-tailed probability test p values: *P ≤ 5 × 10⁻⁴; **P ≤ 5 × 10⁻⁶; ***P ≤ 5 × 10⁻¹²; ****P ≤ 5 × 10⁻³⁰. (D) ChIP-seq data in mid/late gastrulas (8–8.5 h.p.f.) indicating Tbx16 binding within the vgll4l promoter (Bogdanovic et al., 2012; Nelson et al., 2017). Scale is reads per million reads (RPM). Putative T-box binding sites identified using JASPAR are indicated (Fornes et al., 2020).