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Fig. 2

ID

ZDB-FIG-220923-19

Publication

Wattrus et al., 2022 - Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality

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Fig. 2

Fig. 2. Macrophages in the CHT regulate stem cell clonality.

(A) A schematic overview of the Zebrabow-M system: Animals with 15 to 20 insertions of a multicolor fluorescent cassette are crossed to the draculin:CreER^T2 line to enable clonal labeling of lateral plate mesoderm lineages. By treating with 4-hydroxytamoxifen (4-OHT) at 24 hpf just after HSC specification, individual stem cell lineages express specific fluorescent hues that can be quantified in the adult marrow. (B) Families of Zebrabow-M;draculin:CreER^T2 animals injected with either clodronate liposomes or the irf8 morpholino exhibit reduced numbers of HSC clones in the adult marrow, even when macrophages are not depleted until after emergence from the VDA. Data are means ± SD. Data were analyzed by unpaired Student’s t test; *P < 0.05 and ***P < 0.001. (C) Macrophages (mpeg1:EGFP⁺) that have interacted with HSPCs (runx1+23:mCherry⁺) and removed fluorescent material can be harvested by fluorescence-activated cell sorting (FACS). (D) Macrophages that engage HSPCs are marked by lrp1ab and c1qa. The spectral scale reports z-scores. UMAP, uniform manifold approximation and projection.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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