FIGURE

Figure 2

ID

ZDB-FIG-220921-73

Publication

Khrystoforova et al., 2022 - Zebrafish mutants reveal unexpected role of Lrp5 in osteoclast regulation

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Figure 2

lrp5^-/- adult fish display low bone mineral density. (A) Whole-mount alizarin red staining at 1.5 mpf display the distribution of mineralized matrix through the head bones. In the skull of lrp5^-/- distribution of mineralized matrix throughout whole head bones differs from lrp5^+/+ Scale bar=1mm. (B) coronal view of the cranial vault. The alizarin red signal is distributed ubiquitously in lrp5^+/+ lower jaw (C) and opercle (D), meanwhile in lrp5^-/- fish the lower jaw demonstrated discontinues alizarin red signal. Scale bar=500µm. (E) Representative photos of lrp5^+/+ and lrp5^-/- siblings at 4mpf; lrp5^-/- present with deformed head and body curvatures. Scale bar=1cm. (F) 3D reconstruction of X-ray images of lrp5^+/+ and lrp5^-/^- skeleton at 3 and 6mpf. Scale bar= 1cm (G, H) Total BMD is decreased in adult lrp5^-/- fish. (I, J) the skull BMD of lrp5^-/- mutant fish is significantly lower both at 3 and 6mpf. Results in G-J are expressed as mean SD (3mpf n =13-15 fish, 6mpf n =12-13 fish, t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lrp5^{biu102/biu102} (AB)
Observed In:	cranial vault cranial vault bone mineralization head opercle bone mineralization skeletal tissue ventral mandibular arch bone mineralization vertebral column
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)