Fig. 3

Skeletal staining reveals abnormalities in median fin skeletal organization in gdf5^{uu3703/uu3703} zebrafish. Lateral views of cartilage- and bone-stained wild-type fish at 14 dpf (A,A′) and 30 dpf (C,C′,C″); and gdf5^{uu3703/uu3703} fish at 14 dpf (B,B′) and 30 dpf (D,D′,D″). Dashed boxes (A-D) mark magnified regions (A′-D″). (B′) gdf5^{uu3703/uu3703} larvae display separation within the parhypural and hypural 1 cartilage condensations (black asterisks). (D′) Dorsal and anal fin radials are truncated in gdf5^{uu3703/uu3703} fish. The most anterior proximal radials tend to be less affected in both dorsal and anal fin (black arrowheads). This image is from a different mutant fish than displayed in (D). (D″) gdf5^{uu3703/uu3703} fish display truncated and slightly deformed and truncated hemal spines, parhypural and hypural 1. Hypural 3-5 are severely shortened in size (white asterisk). hsp, hemal spine; hy, hypural; nsp, neural spines; phy, parhypural; uro, uroneural. Scale bars: 1 mm (A-D), 100 μm (A′,B′) and 250 μm (C′-D″)