FIGURE

FIG 3

ID

ZDB-FIG-220903-15

Publication

Deng et al., 2022 - AI-2/LuxS Quorum Sensing System Promotes Biofilm Formation of Lactobacillus rhamnosus GG and Enhances the Resistance to Enterotoxigenic Escherichia coli in Germ-Free Zebrafish

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FIG 3

Adherence capacity, biofilm formation and exopolysaccharide (EPS) production of the LGG wild type versus △luxS in the presence or absence of exogenous, synthesized AI-2 (1 μM). (A–B) Adherence of LGG strains to IPEC-J2 cells. Five fields of vision were randomly selected for each slide under oil microscope to calculate the number of bacteria adhered to the surface of the visible cells, n = 6. (C) Biofilm determined by crystal violet staining, n = 6. (D) EPS produced in the biofilm matrix, n = 6. (E-G) Confocal laser scanning microscopy (CLSM) staining of living cells, dead cells, and EPS by SYTO9 (green), PI (red) and calcofluor white (blue), respectively, n = 3. AI-2, autoinducer-2; WT, wild type; ΔluxS, luxS mutant; WT/AI-2, wild-type strain supplemented with AI-2; ΔluxS/AI-2, ΔluxS strain supplemented with AI-2. P < 0.05 (*), P < 0.01 (**) and P < 0.001 (***).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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