Fig. 2

Effect of NYT on aggressive behavior in NPY-KO zebrafish. Wild and NPY-KO zebrafish were fed either control or NYT twice a day for 4 days. Two unfamiliar male zebrafish were set in an aquarium, and their aggressive behavior was analyzed. (A) Test apparatus. (B) Experimental scheme for behavioral evaluation. (C) Tracking of wild (left) and NPY-KO zebrafish (right) swimming behavior. (D) Total number of chases in the WT and NPY-KO zebrafish. (E) Total number of circles in the WT and NPY-KO zebrafish. Wild, n = 6; NPY-KO, n = 7. (F) Total time of aggression in wild and NPY-KO zebrafish. (G) Tracking of control (left) and NYT-fed (right) swimming behavior in NPY-KO zebrafish. (H) Total number of chases in control and NYT-fed NPY-KO zebrafish. (I) Total number of circles in control and NYT-fed NPY-KO zebrafish. (J) Total aggression time in control and NYT-fed NPY-KO zebrafish. n = 6. n.s., not significant. Results are shown as mean ± standard error of the mean.