FIGURE

Fig. 6

ID

ZDB-FIG-220822-23

Publication

Agarwala et al., 2022 - Defining the ultrastructure of the hematopoietic stem cell niche by correlative light and electron microscopy

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Correlative light and electron microscopy (CLEM) Workflow #2 to align all cd41:GFP⁺ hematopoietic stem and progenitor cells (HSPCs) in the larval kidney marrow (KM) niche.

Five days post fertilization (dpf) cd41:GFP⁺ HSPCs (green) and DRAQ5 nuclear dye (blue). (A-D) The same region of the KM niche is marked by a white dotted rectangle. (A) Confocal and brightfield image of thick vibratome section. (B) Confocal Z projection of thick vibratome section. (C) Aligned overlay of micro-computed tomography (microCT) and confocal data. (D) Aligned overlay of serial block-face scanning electron microscopy (SBEM) and confocal data (XY plane only). (E) Aligned overlay of SBEM and confocal data (XY, XZ, YZ planes). (F) Detail of single SBEM section with aligned overlay of cd41:GFP⁺ HSPCs. Anatomical features are labeled and color-coded. (G) Summary of processing steps used in Workflow #2. Abbreviations: D, dorsal; V, ventral; A, anterior; P, posterior.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife