Figure 2

Phosphoproteomic analysis of human neutrophils shows that neratinib treatment induces changes in phosphorylated proteins regulating numerous biological processes. STRING identified interactions between phosphorylated proteins in the combined “DMSO-enriched” and “statistically higher abundance in DMSO” datasets (A) and the combined “neratinib-enriched” and “statistically higher abundance in neratinib” datasets (B). Venn diagrams show the number of phosphopeptides and proteins they map to in each dataset and the overlapping proteins between the two datasets for each treatment group. STRING analysis of these datasets indicates interactions between proteins (lines). STRING also identified a number of Gene Ontology (GO) biological processes that were statistically enriched in both treatment groups, highlighted in color. The neratinib-enriched treatment group had only one statistically enriched biological process, and so statistically enriched keywords from the UniProt database are also shown. Both datasets were searched for the keyword “apoptosis,” and hit proteins were highlighted with a pink outline.