FIGURE
Fig. 2
- ID
- ZDB-FIG-220803-28
- Publication
- Cui et al., 2022 - PARD3 gene variation as candidate cause of nonsyndromic cleft palate only
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Fig. 2
Zebrafish with pard3aa and pard3ab disruption displayed ethmoid plate patterning defects. (A) Schematic diagram of the binding and cleavage site of CRISPR/Cas9 in the coding sequence of the zebrafish PARD3 orthologs (pard3aa, pard3ab). Red = gRNA target. (B) Comparison of craniofacial structures of F0 CRISPR/Cas9-mediated mutant zebrafish and Cas9 control zebrafish. The neurocranium was dissected at 3 dpf. Compared with the larvae in the control group, the CRISPR mutant larvae had a mild dysplasia phenotype, with subtle indentations at the upper edge of the ethmoid plate or hypoplastic at the median ethmoid (arrowheads). me = median ethmoid; le = lateral ethmoid. The statistical analysis of abnormal developmental palate was performed (bars indicate the means ± SEM. Student’s t test, *p < 0.05). Scale bar = 200 μm
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
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| Knockdown Reagents: | |
| Observed In: | |
| Stage: | Protruding-mouth |
Phenotype Detail
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Full text @ J. Cell. Mol. Med.