Fig. 2

Knockout of eaat2a leads to hyperexcitability in response to light stimulation, morphological defects and larval lethality. (a) Lateral view of 5 dpf eaat2a mutants. eaat2a ^?/? larvae (bottom) are slightly curved, do not have an inflated swim bladder and develop pericardial edema (arrowheads). eaat2a ^+/+ (top) and eaat2a ^+/? (middle) larvae are indistinguishable. Scale bar is 500 ?m. (b) Spinal cord length analysis of eaat2a mutants at consecutive days reveals a smaller body size in eaat2a ^?/? (magenta) compared to their eaat2a ^+/? (yellow) and eaat2a ^+/+ (cyan) siblings. Error bars show SD. (c) Mean survival of eaat2a mutants. Error bars show SD. (d) Whole?brain neuronal activity (elavl3:GCaMP6s signal) of three representative eaat2a mutant larvae (eaat2a ^+/+ in cyan, eaat2a ^+/? in yellow and eaat2a ^?/? in magenta) exposed to five 10?second light stimuli with 5?minute interstimulus interval. (e) Changes in fluorescence over time (?F/F₀) per larvae aligned at the onset of light stimuli (red line). (f) ?F/F₀ trace of an example eaat2a ^?/? larva with diverse responses to light?stimuli. (g) Mean responses to five light stimuli per fish in 5 dpf eaat2a ^+/+ and eaat2a ^+/? (cyan, n = 5), and eaat2a ^?/? (magenta, n = 8) zebrafish larvae. Shaded area represents SEM. Significance level: ***p  < .001, **p  < .01, Dunn Kruskal Wallis multiple comparison test (b, c) or Wilcoxon rank?sum test (g). All statistics in Supplementary Table 3