FIGURE

Fig. 8

ID

ZDB-FIG-220720-37

Publication

Berdowski et al., 2022 - Dominant-acting CSF1R variants cause microglial depletion and altered astrocytic phenotype in zebrafish and adult-onset leukodystrophy

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Fig. 8

Elevated lysosomal vesicles in astrocytes and engulfment of myelin debris indicate compensatory astrocytic endocytosis in ALSP. a Representative confocal images of grey matter of the occipital gyrus tissue of late-stage ALSP patients and controls stained with DAPI (nuclei, blue), S100β (astrocytes, green) and LAMP1 (lysosomes, magenta). b, c, d, e Quantification of the number of LAMP1 + S100β + astrocytes in grey matter (b) and white matter (d), and the LAMP1 + area per S100β + astrocyte in grey matter (c) and white matter (e) of the occipital gyrus of late-stage ALSP patients (n = 2) and controls (n = 2). f Representative confocal images showing uptake of MBP + myelin (magenta) by S100β + astrocytes (green) in a white matter lesion in the cingulate gyrus of late stage ALSP patients. Student t test was preformed to test for significance (p < 0.05). Error bars represent SD. *p < 0.05, **p < 0.01 ***p < 0.001. Scale bars equal 50 μm (a) and 30 μm (f)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

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