Fig. 8

rab28 KO zebrafish eyes show a reduced level of total retinoids and retinyl esters at 15 days post fertilization (dpf) with an additional reduction in levels of the visual chromophore 11-cis-retinal at 8 months post fertilization (mpf). (A) Retinoid profiles of 15 dpf rab28 KO zebrafish eyes and sibling controls were analyzed using normal phase high-performance liquid chromatography (HPLC). Bars represent the mean ± SD of three independent experiments for each genotype with 100 heads per biological replicate. rab28 KO mutant eyes showed significantly reduced levels of 11-cis-retinyl palmitate (p = .0127), all-trans-retinyl palmitate (p = .0306) compared with sibling controls (B) Retinoid profiles of 8 mpf rab28 KO zebrafish eyes and sibling controls were analyzed using HPLC. Bars represent the mean ± SD of three independent experiments for each genotype with four eyes per biological replicate. rab28 KO eyes show significantly reduced levels of 11-cis-retinal (p = .0110), all-trans-retinyl palmitate (p = .0313) compared with sibling controls. rab28 KO eyes also show a significantly reduced level of total retinoid (p = .0178). (C) Bisretinoid profiling by HPLC reveal a significant increase (p = .0059) in the levels of A2PE in the rab28 KO compared with wild-type sibling controls at 1 year old. Bars represent the mean ± SD of A2PE expressed as milli absorbance units (mAU); six eyes per biological replicate (n = 4) were analyzed for each genotype. Data were analyzed by unpaired, two-tailed t tests