FIGURE

Fig. 8

ID

ZDB-FIG-220715-13

Publication

Liu et al., 2022 - Cre/lox regulated conditional rescue and inactivation with zebrafish UFlip alleles generated by CRISPR-Cas9 targeted integration

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Fig. 8

(A) Diagram of expected Cre-mediated inversion of rb1^off to on orientation. (B, C) pH3 and HuC/D labeling of larval sectioned head tissue from 5 dpf transheterozygous rb1^off/stop (B ? B?) and Cre injected rb1^off/stop (C ? C?). (D, E) pH3 and Cre labeling of larval sectioned head tissue from 5 dpf transheterozygous rb1^off/stop (D ? D?) and neurod1-2A-Cre; rb1^off/stop (E ? E?). (F) Quantification of pH3-positive cells in control rb1^off/stop (n=3) and Cre injected rb1^off/stop (n=3) midbrain (** p<0.01) and retina (** p<0.01). (G) Genomic DNA qPCR quantification of rb1^off original orientation DNA 5? and 3? junctions in control rb1^off/stop (n=3) and Cre injected rb1^off/stop (n=3). (H) Quantification of pH3-positive cells in rb1^off/stop (n=3) and neurod1-2A-Cre; rb1^off/stop (n=3) midbrain (** p<0.01) and retina (* p<0.05). (I) Genomic DNA qPCR quantification of rb1^off original orientation DNA 5? and 3? junctions in control rb1^off (n=3) and neurod1-2A-Cre; rb1^off/stop (n=3). Error bars represent mean � s.e.m. with two-tailed t-test. Scale bars: 50 ?m (B - E), 10 ?m (B? ? E?).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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