Fig. 1

a Stable transgenic rag2:RFP and rag2:IL7R^mut2-tdTomato zebrafish were followed for disease onset and progression. Representative images of stable transgenic zebrafish at 17 weeks of life. Panels are merged fluorescent and brightfield images. Scale bar, 2?mm. b Kaplan?Meier analysis of disease progression in stable transgenic zebrafish (Gehan-Breslow-Wilcoxon statistic). Number of animals analyzed per genotype is shown in parenthesis. c May-Grünwald and Wright-Giemsa stained cytospins of kidney marrow from wild-type fish and bulk leukemias of rag2:IL7R^mut2-tdTomato fish (left panels); Scale bar, 50?µm. Histological analysis of thymic cells from wild-type fish (n?=?4) and primary T-ALLs (n???6); Hematoxylin and eosin-stained sections juxtaposed to immunohistochemistry for TUNEL (right panels). Arrowheads denote examples of positively stained cells. Scale bar equals 10?µm. Percent positive cells?±?SEM are shown within each image panel. Asterisks denote significant differences as assessed by Student?s t test. d Immunoblot analysis of phosphorylated protein levels in normal rag2:RFP thymocytes and bulk leukemias or FACS-sorted T-ALL cells from stable transgenic animals (n?=?8).