ZFIN ID: ZDB-FIG-220628-43
Wiltbank et al., 2022 - Cd59 and inflammation regulate Schwann cell development. eLIFE   11 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Prim-25 to Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Days 7-13

Fig. 4

(A) Immunofluorescence (IF) showing Sox10-positive SCs (black/gray) along the posterior lateral line nerve (pLLN) from 36 hours post fertilization (hpf) to 7 days post fertilization (dpf). Black dashed lines outline the pLLN. Sox10-positive pigment cells outside of the dashed lines (white asterisks) were not included in the analysis. (B–E) Scatter plots of the number of Sox10-positive SCs along the pLLN from 36 hpf to 7 dpf (mean ± SEM: 36 hpf: WT: 5.6 ± 0.2, cd59uva48: 9.1 ± 0.3; 48 hpf: WT: 8.6 ± 0.5, cd59uva48: 15.0 ± 0.7; 72 hpf: WT: 18.0 ± 0.4, cd59uva48: 24.3 ± 0.8; 7 dpf: WT: 27.0 ± 0.6, cd59uva48: 33.9 ± 0.8; p-values: p<0.0001; dot = 1 fish). (F) EdU incorporation assay showing sox10:tagrfp-positive, pLLN SCs (orange) pulsed with EdU (cyan) from 48 to 55 hpf. (G) Scatter plot of the number of EdU-positive SCs along the pLLN at 55 hpf (WT: 6.7 ± 0.5, cd59uva48: 13.6 ± 0.5; p<0.0001; dot = 1 fish). (H) Scatter plot of the number of mitotic events observed in SCs from 48 to 55 hpf (mean ± SEM: WT: 0.6 ± 0.1, cd59uva48: 1.7 ± 0.3; p=0.0019; dot = 1 fish). (I) Scatter plot of the number of mitotic events observed in SCs from 54 to 75 hpf (mean ± SEM: WT: 4.1 ± 0.3, cd59uva48: 4.1 ± 0.4; dot = 1 fish). (H) Scatter plot of the time of final cell division (hpf) observed in SCs from 54 to 75 hpf (mean ± SEM: WT: 69.1 ± 1.1, cd59uva48: 68.4 ± 0.9; dot = 1 fish). All data were collected from somites 11–13 (~320 µm) and normalized to units per 100 µm. All images in this figure were acquired with confocal imaging. Each dataset was compared with Student’s t-test using GraphPad Prism. Scale bars: (A, F) 25 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
sox10 Ab2-sox10 AB standard conditions Prim-25 posterior lateral line nerve immature Schwann cell IHC
Ab2-sox10 Long-pec posterior lateral line nerve myelinating Schwann cell IHC
Ab2-sox10 Protruding-mouth posterior lateral line nerve myelinating Schwann cell IHC
Ab2-sox10 Days 7-13 posterior lateral line nerve myelinating Schwann cell IHC
Ab2-sox10 cd59uva48/uva48 standard conditions Prim-25 posterior lateral line nerve immature Schwann cell IHC
Ab2-sox10 Long-pec posterior lateral line nerve myelinating Schwann cell IHC
Ab2-sox10 Protruding-mouth posterior lateral line nerve myelinating Schwann cell IHC
Ab2-sox10 Days 7-13 posterior lateral line nerve myelinating Schwann cell IHC
TagRFP cd59uva48/uva48; uva5Tg standard conditions Long-pec posterior lateral line nerve myelinating Schwann cell IHC
uva5Tg standard conditions Long-pec posterior lateral line nerve myelinating Schwann cell IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cd59uva48/uva48 standard conditions Prim-25 posterior lateral line nerve has extra parts of type posterior lateral line immature Schwann cell, abnormal
Long-pec posterior lateral line nerve has extra parts of type posterior lateral line myelinating Schwann cell, abnormal
Protruding-mouth posterior lateral line nerve has extra parts of type posterior lateral line myelinating Schwann cell, abnormal
Days 7-13 posterior lateral line nerve has extra parts of type posterior lateral line myelinating Schwann cell, abnormal
cd59uva48/uva48; uva5Tg standard conditions Long-pec posterior lateral line nerve Schwann cell proliferation increased occurrence, abnormal
Acknowledgments:
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