FIGURE

Figure 7

ID

ZDB-FIG-220520-8

Publication

Duclaux-Loras et al., 2022 - UNC45A deficiency causes microvillus inclusion disease-like phenotype by impairing myosin VB-dependent apical trafficking

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Figure 7

MVID features in enterocytes of <italic toggle='yes'>unc45a</italic> zebrafish mutants.

(A–C) Confocal microscopy analysis of the intestinal bulb of WT and unc45a^–/– mutant larvae stained for villin and pERM (A), F-actin (phalloidin) (B), and Rab11 (C) at 5 dpf. Scale bars: 20 μm. Boxes showing microvillus-like inclusions are enlarged ×4. (D and E) TEMs of thin sections of intestinal bulb of 5 dpf WT and unc45a^–/– mutant larvae showing defects in the organization of the brush border. Scale bars: 2 μm (D); 0.5 μm (E). Quantitative analysis showing decrease in microvillus length and density in unc45a^–/– enterocytes as compared with WT enterocytes. Data are represented as mean + SD. ****P < 0.001, t test. N nucleus; MV microvilli.

Expression Data

Antibodies:	Ab1-villin Ab2-rab11
Fish:	WT unc45a^tr12/tr12
Anatomical Terms:	enterocyte apical plasma membrane enterocyte vesicle intestinal epithelial cell basolateral plasma membrane
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	unc45a^tr12/tr12
Observed In:	enterocyte apical plasma membrane enterocyte microvillus intestinal epithelial cell basolateral plasma membrane
Stage:	Day 5

Phenotype Detail

Acknowledgments

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