Figure 4

Figure 4. Swimming behaviour of 120 hpf zebrafish larvae exposed to lovastatin (LOV) and L-carnitine (LOV+LC200) treatment. (A) Spontaneous displacement of zebrafish larvae in control (non-treated, NT; vehicle control, VC; 100 ?M L-carnitine, LC100; 200 ?M L-carnitine, LC200) and experimental (0.5 ?M lovastatin, LOV; 0.5 ?M lovastatin and 100 ?M L-carnitine, LOV+LC100; 0.5 ?M lovastatin and 200 ?M L-carnitine LOV+LC200) groups. Graphs show larvae displacement over the 10 min period. (B) Bar graph demonstrates the percentage of zebrafish larvae in seven investigated groups in 4 ranges of the distance travelled: no movement (up to 0.5), from 0.5 to 1.5, from 1.5 to 3, and more than 3 cm. The table below indicates the pairwise comparison between responses of seven investigated groups. Statistically significant differences are indicated with *; * p < 0.05 (Kruskal?Wallis test followed by the Pairwise Mann?Whitney post-hoc test). The experiment was repeated at least 3 times (with 18 to 20 individuals in each investigated group). (C) Touch-evoked response assay. Stacked column graphs demonstrate the number of zebrafish larvae as a function of their response to first (F) and second (S) tail touch stimulation, as well as in response to a head touch. Depending on the type of reaction, zebrafish larvae were divided into 4 groups: no response, response without escaping (defined as a lateral undulation of the tail immediately after being touched), short distance (larvae travelled a distance of no more than 20 mm), long distance (larvae travelled more than 20 mm). The tables below indicate the pairwise comparison between responses of seven investigated groups. Statistically significant differences are indicated with *; * p < 0.05 (Kruskal?Wallis test followed by the pairwise Mann?Whitney post-hoc test). The experiment was repeated at least 3 times (with 18 to 20 individuals in each investigated group).