FIGURE 1

Experimental design timeline for different life stages. (A) Zebrafish embryos up to 4 h post-fertilization (hpf) were exposed to water (control groups) or to different concentrations of the two herbicides (atrazine or diuron) for 96 h, after which they were transferred to an environment free of herbicides in which they remained until 7 days post-fertilization (dpf). During 7 dpf, morphological parameters, survival, and hatching rates were daily monitored. At 7 dpf, behavioral analyzes were performed and included the exploration of a new environment, aversive behavior, and optomotor response and individuals were photographed. (B) Adult animals aged 12–18 months were exposed to water (control groups) or to different concentrations of atrazine or diuron. Animals were exposed for 96 h, after which they were transferred to an environment free of herbicides where they remained for more than 3 days. On the third day, their performance on a new environment and the social interaction were evaluated. In the following day, an analysis of aggressive behavior was performed.