Figure 1

Tricaine onset and recovery in non-restrained zebrafish larvae. (A) Larvae were placed in dishes for 20 min to acclimatize (referred to as baseline), followed by 7 min of tricaine exposure. After these 7 min, larvae were transferred to a dish containing tricaine-free E3 and recovery time was assessed. Time to loss of tactile response (B), loss of righting reflex (C), and recovery (D) of tactile response were measured in larvae exposed to three concentrations of tricaine: the standard dose (168 mg/L), half this concentration, and double this concentration. Tricaine onset and recovery was dose-dependent, with lower concentrations having the slower onset and faster recovery times. Box plots show quartiles; whiskers extend to the most extreme data points not considered outliers (within 2.7 standard deviations). Results were analyzed via one-way ANOVA with Tukey's HSD test. N = 8 for all three conditions, *p < 0.05, **p < 0.005, ***p < 0.001.