Figure 4.

Egr1 is necessary for TH2 expression. A, Western blotting of larval head homogenates at 14 dpf reveals a reduction in expression of TH2 in egr1 mutants. A’, Two color labeling of a Western blotting to highlight in-lane normalization to β-actin and technical replicates. B–E, Western blot intensities in A, A’ were first normalized to in-lane β-actin, and then to wt average intensity. N = 4 experiments, n = 2 technical replicates, *p < 0.05, HSD. F, Immunolabeling of synapses with antibodies to Synapsin 1 and 2 (Syn1/2, magenta) and Gephyrin (Geph, green) in the anterior commissure (AC), lateral view. Insets are magnifications of the cyan box. Scale bar: 5 μm. G, Quantification of synaptic puncta revealed no changes in synapse density in the medial and lateral AC. n ≥ 5 brains per genotype. wt (+/+, white), het (+/−, light gray), mut (−/−, dark gray). Error bars are SEM.