Fig. 7.
Early paralysis reduces numbers of entpd5a-expressing pre-hypertrophic chondrocytes in ch cartilage. (A-D) Live imaging of wild-type control (A,B) and ?-BTX-injected paralyzed (C,D) sox10:lyn-tdTomato;entpd5a:kaede double-transgenic embryos at 96 hpf. The HZ does not develop in ?-BTX-injected animals. (A,C) Optical slices. (B,D) z-projections. White arrowheads indicate the position of the HZ. (E) Quantification of number of entpd5a:kaede-labeled chondrocytes in the ch cartilage. ch, ceratohyal; D, dorsal; hs, hyosymplectic; pq, palatoquadrate; V, ventral. All micrographs are ventral views with anterior to the left. Scale bar: 50 ?m (A-D). |