FIGURE

Figure 4

ID

ZDB-FIG-220330-10

Publication

Nielson et al., 2022 - Glyoxylate protects against cyanide toxicity through metabolic modulation

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Figure 4

Glyoxylate’s rescue mechanism is not fully explained by cyanohydrin formation. Dose dependent survival of 5 dpf zebrafish in the presence of (A) scavengers (3 fish per well, N = 6 wells for each condition) or (B) cyanohydrin-forming molecules 20 h after exposure to 20 µM KCN (3 fish per well, N = 6 wells for each condition with the exception of alpha-ketobutyrate, N = 3). Data presented as mean ± SEM. HCP was 100% lethal at the highest dose (1 mM) and this data point was removed to preserve an accurate dose–response curve. (C) Continuous-wave near-infrared spectroscopy (CWNIRS) monitoring changes in concentrations of deoxygenated (RHb), oxygenated (OHb), and total (THb) hemoglobin. The rabbits were exposed to a sublethal dose of 10 mg i.v. NaCN for 60 min. At 60 min, subjects received equimolar doses of glyoxylate or (D) alpha-ketoglutarate (100 mg or 147 mg respectively). Results shown are typical of the response seen by CWNIRS in animals treated with cyanide and glyoxylate.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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