Fig. 3

Starved zebrafish differentially regulate intestinal gene expression when compared to fed zebrafish. A Principal Components Analysis (PCA) of RNA-Seq libraries in starved/refed and fed control zebrafish intestines at 3dpS, 21dpS, and 3dpR. B Quantification of the number of significantly upregulated and downregulated genes in starved/refed zebrafish intestines at each timepoint. Note that these numbers in panel B include genes that were also significantly differential in our fed control comparisons. C Hierarchical clustering of log2 fold changes in gene expression in starved zebrafish intestines, along with flattened values that show significant changes in gene expression, and z-scores based on normalized counts of each gene. D Log2 fold changes in gene expression in starved zebrafish intestines at 21dpS when compared to 21dpS fed fish plotted according to their -log₁₀ adjusted p-values. Note that data plotted in panels C and D do not include genes that were significantly differential within fed fish (see Table S3B and Fig. S2). E UCSC tracks of representative replicates show that elovl2 mRNA, encoding a fatty acid elongase, is downregulated in starved zebrafish intestines and returns to levels comparable to the fed group upon re-feeding. F UCSC tracks of representative replicates show that tmprss15 mRNA, encoding an enteropeptidase, is upregulated in starved zebrafish intestines and returns to levels comparable to the fed group upon re-feeding