FIGURE

Figure 1—figure supplement 2.

ID

ZDB-FIG-220324-33

Publication

Saraswathy et al., 2022 - The E3 Ubiquitin Ligase Mindbomb1 controls planar cell polarity-dependent convergent extension movements during zebrafish gastrulation

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Figure 1—figure supplement 2.

Axis extension and RNA expression in different <italic toggle='yes'>mib1</italic> mutants.

(A,B) Analysis of bud stage axis extension (A) and mib1 in situ hybridization (B) show that the mib1^ta52b mutation does not impair Convergent Extension (CE) or mib1 transcript abundance (B, n = 4 mutant embryos analyzed). (C,D) In contrast mib1^nce2a mutants present a weak reduction of CE (C) and a loss of mib1 transcripts (D, n = 6 mutant embryos). (E) qPCR analysis of mib1 transcript levels (30 hfp stage). mib1^tfi91 and mib1^nce2a homozygous mutants present a reduction in mib1 transcripts that is not observed for the mib1^ta52b allele. Error bars represent SE from three biological replicates. (F) mib1^nce2a mutants present a CrisprCas-induced InDel that causes a frame shift and leads to premature protein truncation. (A,C) depict lateral views of bud stage embryos, anterior up, dorsal to the right. A quantitative analysis of the corresponding data sets is provided in Figure 1D and G. (B,D) Represent dorsal views of bud stage embryos, anterior up. To warrant identical acquisition conditions, two embryos were photographed on a single picture. Scalebars: 200 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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