FIGURE

Fig. 3.

ID

ZDB-FIG-220317-10

Publication

Serrano et al., 2021 - Novel pre-clinical model for CDKL5 Deficiency Disorder

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Fig. 3.

Assessment of neuronal pathology in cdkl5^−/− fish. (A) Maximum intensity projection confocal images of EGFP-labelled motor neurons from Tg(islet1:EGFP); cdkl5^+/+ (white dashed box zoomed in i) and Tg(islet1:EGFP); cdkl5^−/− (white dashed box zoomed in ii) along the spinal region at 6 dpf. Red dashed boxes indicate the regions quantified in C. Scale bars: 50 µm. (B) Quantification of the number of EGFP⁺ motor neurons at 6 dpf. Data are mean±95% c.i. for three independent experiments [n=8, 8 and 8 for Tg(islet1:EGFP); cdkl5^+/+, and n=8, 8 and 8 for Tg(islet1:EGFP); cdkl5^−/− fish]. (C) Quantification of the mean percentage area of fluorescence from Tg(islet1:EGFP); cdkl5^+/+ and Tg(islet1:EGFP); cdkl5^−/− (within the red dashed box region in A) at 6 dpf. Data are mean±s.e.m. for three independent experiments [n=8, 8 and 8 for Tg(islet1:EGFP); cdkl5^+/+, and n=8, 8 and 8 for Tg(islet1: EGFP); cdkl5^−/− fish]. **P<0.01; NS, not significant (two-tailed t-test).

Expression Data

Gene:	GFP
Fish:	rw0Tg cdkl5^{sa21938/sa21938}; rw0Tg
Anatomical Terms:	spinal accessory motor neuron cell body spinal accessory motor neuron neuron projection
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rw0Tg cdkl5^{sa21938/sa21938}; rw0Tg
Observed In:	spinal accessory motor neuron spinal accessory motor neuron neuron projection
Stage:	Day 6

Phenotype Detail

Acknowledgments

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