FIGURE
Figure 1
- ID
- ZDB-FIG-220314-50
- Publication
- Molagoda et al., 2022 - Fermented Oyster (Crassostrea gigas) Extract Cures and Prevents Prednisolone-Induced Bone Resorption by Activating Osteoblast Differentiation
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Figure 1
Figure 1. FO promotes osteogenic activity in prednisolone (PDS)-pretreated MC3T3-E1 cells. Mouse preosteoblast MC3T3-E1 cells (1 × 104 cells/mL) were pretreated with 10 ?M PDS for two days prior to treatment with FO (0?200 ?g/mL) for five days. Fresh media with FO and/or PDS were replenished every two days. At day 7, (A) bone mineralization was evaluated by alizarin red staining, and (B) ALP activity was evaluated using a TRACP & ALP Double-Staining Kit. (C) In a parallel experiment, total mRNA was extracted, and RT-PCR was performed to evaluate the gene expressions of ALP, RUNX2, and OSX. GAPDH was used as the internal control. (D) Total proteins were extracted, and Western blotting was performed to evaluate the expression of ALP, RUNX2 and OSX proteins. ?-Actin was used as the internal control. All data are presented as means ± standard error of the mean (### p < 0.001 vs. untreated MC3T3-E1 cells; * p < 0.05 and *** p < 0.001 vs. PDS-treated MC3T3-E1 cells). ALP: alkaline phosphatase; RUNX2: runt-related transcription factor 2; OSX: osterix; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
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