FIGURE

Fig. 2.

ID

ZDB-FIG-220305-53

Publication

Gora et al., 2021 - Analysis of the H-Ras mobility pattern in vivo shows cellular heterogeneity inside epidermal tissue

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Fig. 2.

Schematic overview of SMM applied to a living zebrafish embryo. A zebrafish embryo was injected with an expression vector for a YFP fusion protein shortly after fertilization. At 2 dpf, it was placed on a coverslip coated with poly-L-lysine in a drop of egg water. The tail region of the embryo was covered with a 0.75-mm-thick agarose (2%) sheet. On the lower left part of the figure, an epifluorescence picture is presented of the outer layer of the epidermis, showing the fluorescent signal of YFP-C10H-Ras in the cell membranes. Morphologically, the cells in this layer are homogenous and are characterized by a hexagonal shape. On the lower right part, a TIRFM image of an area within the field of view of the epifluorescence picture is presented, with examples of individual YFP molecules shown in circles. Scale bars: 5 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.