FIGURE

Figure 2

ID

ZDB-FIG-220302-79

Publication

Vagionitis et al., 2022 - Clusters of neuronal neurofascin prefigure the position of a subset of nodes of Ranvier along individual central nervous system axons in vivo

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Figure 2

Clusters of neuronal neurofascin are stable over time and do not co-localize with processes of oligodendrocyte precursor cells

(A) Individual unmyelinated axon expressing Nfasca-EYFP at two different time points. Arrowheads depict stable clusters and the arrow points to a cluster that disappears between time points. Scale bar, 20 μm.

(B) Quantification of Nfasca cluster and node motility. Median ± IQR (Mann-Whitney test); n = 5/5 (cluster motility) and 9/6 (node motility) animals/technical replicates.

(D) Axon expressing Nfasca-EYFP in a transgenic animal that has all OPCs labeled. Scale bar, 50 μm. (D′) (Left) Magnification of the boxed area in (D) showing OPC processes in relation to Nfasca-EYFP accumulations over time. Scale bar, 10 μm. (Right, i–iii) Examples of possible cluster-OPC process interactions. Scale bar, 2 μm.

(E) Quantification of the frequency of cluster-OPC process interactions; n = 4/2 animals/technical replicates.

See also Figure S2 for supporting information.

Expression Data

Genes:	mScarlet YFP
Fish:	tum101Tg; ue601Tg tum111Tg; ue601Tg
Anatomical Terms:	neuron node of Ranvier oligodendrocyte
Stage Range:	Day 4 to Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	tum111Tg; ue601Tg
Observed In:	neuron node of Ranvier oligodendrocyte
Stage:	Day 4

Phenotype Detail

Acknowledgments

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