Figure 2

ACY-1215 demonstrates anti-cancer effects in vivo in zebrafish OMM2.5 xenografts. (A): Schematic depicting the workflow for assessing ACY-1215 effects in vivo. (B): Top panel shows representative images of Dil-labeled OMM2.5 cells transplanted into the perivitelline space of 2-day-old zebrafish larvae. Bottom panels present representative images of the distribution of OMM2.5 Dil-labeled cells in xenografts 3 days post treatment (dpt), with 0.5% DMSO (n = 30), 20 μM ACY-1215 (n = 31) or 20 μM Dacarbazine (n = 27). Days post fertilization (dpf). (C): At 3 dpt, OMM2.5 Dil-labeled cells have disseminated (white arrowhead) to the caudal vein plexus of the zebrafish larvae. (D): ACY-1215 treatment for 3 days resulted in a significant (****, p = 0.0001) reduction in normalized primary xenograft fluorescence on average in comparison to larvae treated with 0.5% DMSO or 20 μM Dacarbazine. (E): There was no observed difference in the average number of disseminated cells between vehicle control-treated or drug-treated groups after 3 days. Statistical analysis was performed using one-way ANOVA with Dunnett’s Test for Multiple Comparisons, and error bars present mean ± SEM.