FIGURE

Fig. 5A

ID

ZDB-FIG-220218-7

Publication

Wu et al., 2021 - Inhibition of endoplasmic reticulum stress-related autophagy attenuates MCLR-induced apoptosis in zebrafish testis and mouse TM4 cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5A

MCLR activates ER stress and autophagy in TM4 cells. (A) Representative immunoblots showed GRP78 proteins after different treatment. GAPDH was used as the control. Relative protein levels of GRP78 were shown from 4 independent experiments. (B) The mRNA expression of grp78 were evaluated by qRT-PCR (n = 4). (C) The mRNA expression of autophagy related genes atg5, atg12 and lc3b were evaluated by qRT-PCR (n = 4). (D) The expression levels of the autophagy markers ATG5, ATG12, LC3BⅠ and LC3BⅡ were analyzed by Western blot (n = 3). (E) Cell imaging was performed to analyze the colocalization of LysoTracker-stained acidified vesicles and LC3b-positive autophagosomes in control and MCLR-treated cells. Scale bar: 50 µm. *P < 0.05 significantly different from control group. ^P < 0.05 significantly different from 7.5 μg/mL MCLR. ▲P < 0.05 significantly different from 30 μg/mL MCLR.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Ecotoxicol. Environ. Saf.