FIGURE

Figure 3

ID

ZDB-FIG-220213-16

Publication

Swain et al., 2022 - Construction and Evaluation of Recombinant Attenuated Edwardsiella piscicida Vaccine (RAEV) Vector System Encoding Ichthyophthirius multifiliis (Ich) Antigen IAG52B

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Figure 3

Complementation of ΔasdA mutant strains with E. piscicida AsdA⁺ Plasmids. (A)E. piscicida AsdA⁺ Plasmid maps (pG8R8011-pG8R8018) showing origin of replication, orientation of asdA gene, presence or absence of the promoter for the asdA gene and with either ATG or GTG start codons, P_trc promoter and multiple cloning sites. (B) Characteristic of E. piscicida AsdA⁺ plasmids or various strategies to construct AsdA⁺ plasmids via modification of the plasmid copy number (pUC ori and pBR ori), promoter, SD sequence and start codon. (C) Growth of ΔasdA (χ16000) mutant strains complemented with different E. piscicida AsdA⁺ plasmids (pG8R8011 - pG8R8018).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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