FIGURE

Fig. 5

ID

ZDB-FIG-220204-13

Publication

Hong et al., 2020 - In Situ Fucosylation of the Wnt Co-receptor LRP6 Increases Its Endocytosis and Reduces Wnt/β-Catenin Signaling

Other Figures

All Figure Page

Back to All Figure Page

Fig. 5

(A) Diagram summarizing our new findings and predicting the presence of a Fuc-binding receptor. Increasing cell-surface N-glycan LacNAc ?(1?3)-fucosylation via ISF treatment exacerbates the endocytosis of the lipid-raft-localized Wnt receptor LRP6 which, in turn, leads to reduced Wnt/?-catenin signaling. The majority of endocytosed cell-surface LRP6 enters the recycling pathway rather than the degradation pathway following endocytosis induced by ISF treatment. (B) The chemical structure of four monosaccharides in a vertebrate N-glycan: L-fucose (Fuc), D-glucose (Glc), D-galactose (Gal), and D-mannose (Man). (C and D) Wnt-signaling activity in Pro-5 cells treated by ISF or FucT only, in the presence (C) or absence (D) of a free monosaccharide. Error bars represent SE of three biological replicates. Unpaired two-tailed Student's t test: ?p < 0.05, ??p < 0.01; NS, not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Chem Biol