FIGURE

Fig. 2

ID

ZDB-FIG-220203-85

Publication

Zhou et al., 2021 - Corticotropin-releasing hormone reduces basal estradiol production in zebrafish follicular cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Regulation of steroidogenic gene expression levels and E2 content in zebrafish follicular cells in vitro by CRH. (A and C) Treating primary cultured follicular cells with different concentrations of CRH? or CRH? for 24 h mRNA expression of cyp19a1a, hsd17b1, and hsd17b3 were detected using qPCR, respectively. (B and D) Treating primary cultured follicular cells with 100 nM CRH? or CRH? for 1.5?24 h. (E and F) Primary cultured follicular cells incubated with various concentrations of CRH? or CRH? for 24 h, or with 100 nM CRH? or CRH? for 1.5?24 h. The content of E2 in the medium was measured by ELISA. The data were represented as the mean � SEM (n = 4). Different lowercase above columns indicates statistical significance (P < 0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Molecular and Cellular Endocrinology, 527, Zhou, H., Chen, A., Lu, W., Corticotropin-releasing hormone reduces basal estradiol production in zebrafish follicular cells, 111222, Copyright (2021) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.