Fig. 5

Kinase-dead Bmpr1 can restore Bmp signaling in Bmpr1-deficient embryos: (A and B) The bubble-plot fill color reflects genotype condition. Raw phenotype scores are in Dataset S4. (A) Kinase-dead Bmpr1 can rescue bmpr1aa−/−; bmpr1ab−/− double mutant embryos. Column 1: uninjected bmpr1aa+/+; bmpr1ab−/− and bmpr1aa+/−; bmpr1ab−/− embryos are phenotypically WT. Columns 2-4: bmpr1aa+/−; bmpr1ab−/− embryos injected with WT bmpr1aa RNA (40 to 80 pg) (column 2), kinase-dead bmpr1aa-K256R RNA (20 to 80 pg) (column 3), or GS-domain mutant bmpr1aa-VVAAA RNA (80 pg) (column 4). Column 5: bmpr1aa−/−; bmpr1ab−/− double mutants are C4 dorsalized. Columns 6 to 8: bmpr1aa−/−; bmpr1ab−/− double mutants injected with WT bmpr1aa RNA (column 6), bmpr1aa-K256R RNA (column 7), or bmpr1aa-VAAA RNA (column 8). (B–F) Representative-rescued embryos from bmpr1 genotypes and injection conditions in the following: a bmpr1aa+/−; bmpr1ab−/− embryo (A and B), bmpr1aa−/−; bmpr1ab−/− double mutants (C–F), uninjected (C), injected with WT bmpr1aa RNA (D), injected with bmpr1aa-K256R RNA (E), and injected with bmpr1aa-VAAA RNA (F). (G) Kinase-dead Bmpr1 rescues the simultaneous depletion of all four zebrafish Bmpr1 receptors. Experimental conditions in all columns are as in A, except that all embryos are additionally injected with a combination of bmpr1ba and bmpr1bb MO. MO concentrations and combinations are listed in SI Appendix, Table S4. H–L is the same as B–F but with additional bmpr1ba and bmpr1bb MO injection.