Fig. 3

dtymk knockout zebrafish. a Genomic location of the gRNA sequence used to generate the knockout line of dtymk, indicated in yellow. The mutant line, Δex4 contains a 5-bp deletion in exon 4 resulting in a truncated protein. b Phenotype of dtymk knockout (dtymk –/–) embryos at 5dpf and their siblings (sib). The homozygous mutant embryos (dtymk –/–) show small, irregular eyes and edema around the brain, heart and intestine. The scale bar represents 200 μm. c Hematoxylin/eosin staining of transversal sections through the head of sibling and dtymk mutant embryos at 3dpf and 5dpf at the level, where the optic nerve exits the eye. Images show prominent degeneration of brain tissue (blue star) and structural abnormalities of the eyes and jaw cartilage structures (blue arrow) in the mutant embryos (dtymk –/–) which is absent in the sibling (sib) embryos. The scale bar represents 100 μm. d Quantification of head size in dtymk –/– and sibling (sib) embryos at 2dpf. Average head size of 19–24 embryos at 2dpf showing significant microcephaly in mutant vs. sibling embryos (***, p = 1.52 × 10^–13). Error bars show the standard deviation. Two biological replicates. The scale bar represents 200 μm