Fig. 1

(A) Serum endotoxin in LFD-, LFSP0.5-, HFD-, and HFSP0.5-fed zebrafish at the end of the 2-week feeding trial (n = 4). (B and C) The relative mRNA expression of (B) claudin-15 and (C) occludin in the intestine of LFD-, LFSP0.5-, HFD-, and HFSP0.5-fed zebrafish at the end of 2-week feeding trial (n = 5–6). (D) Representative histopathologic image of H&E-stained intestinal sections. The scale bar, 50 μm. (E) Histological score measuring the severity of the intestinal damage of zebrafish (n = 5). (F–H) (F) Caspase-9, (G) caspase-6, and (H) caspase-3 activities in the intestine of 1-month-old zebrafish fed the LFD, HFD, or HFSP0.5 for 2 weeks (n = 4–6). Values are means ± SEM. Means without a common letter are significantly different (p < 0.05). Duncan's test. LFD, low-fat diet; LFSP0.5, 0.5% propionate-supplemented LFD; HFD, high-fat diet; HFSP0.5, 0.5% propionate-supplemented HFD.