Figure 4
- ID
- ZDB-FIG-211219-71
- Publication
- Rondelli et al., 2021 - The ubiquitous mitochondrial protein unfoldase CLPX regulates erythroid heme synthesis by control of iron utilization and heme synthesis enzyme activation and turnover
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CLPX regulates the terminal steps of heme synthesis.A, ALAS2 activity was significantly increased in Clpx−/− and Clpp−/− cells. B, Clpx−/− and Clpp−/− cells produced significantly more ALA than WT cells. C, Clpx−/− and Clpp−/− cells produced significantly more PPIX than WT cells. D, PPOX protein levels were decreased in undifferentiated Clpx−/− and Clpp−/− cells but not appreciably in differentiated cells. However, FECH protein levels were decreased, N = 3. E, PPOX activity was decreased in Clpx−/− cells but was unaltered in Clpp−/− cells. F, FECH activity was decreased in undifferentiated Clpx−/− cells, relative to wild-type cells. The FECH activity of Clpp−/− cells was unaltered. In differentiating MEL cells, FECH activity was decreased in both Clpx−/− and Clpp−/− cells. ∗p < 0.05. Error bars indicate SD. |