FIGURE

Figure 3

ID

ZDB-FIG-211120-42

Publication

Ding et al., 2021 - The chromatin-remodeling enzyme Smarca5 regulates erythrocyte aggregation via Keap1-Nrf2 signaling

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Figure 3

TEM shows abnormal organelle morphology in smarca5-deficient RBCs.

(A) The transmission electron microscopy (TEM) view of a longitudinal section through the artery and vein plexus in sibling tail region at 2 dpf. (B) TEM view of erythrocytes in sibling embryos. The red arrow heads indicate the characteristic pattern of organization in mitochondria. (C) TEM view of a longitudinal section through the artery and vein plexus in smarca5^zko1049a tail region at 2 dpf. (D) The blue rectangular box showing blood clots in the caudal vein plexus in smarca5^zko1049a. The magnification of blood clots is shown (right). (E) TEM view of erythrocytes in smarca5^zko1049a. The red arrow heads indicate the characteristic pattern of organization in mitochondria. The disintegration of cristae in mitochondria is observed in smarca5-deficient RBCs. Ery, erythrocyte; EC, endothelial cell; Mito, mitochondria; Nuc, nucleus; Cyto, cytoplasm. (F) Scatter plots showing the section area of mitochondria relative to cell in RBCs from smarca5^zko1049a and their siblings. (G) Scatter plots showing the number of mitochondria per cell section in RBCs from smarca5^zko1049a and their siblings. Data are mean ± s.d. (F and G). Asterisk presents statistical significance (n.s. not significant). p Values were calculated by two-tailed unpaired Student’s t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	smarca5^{zko1049a/zko1049a} smarca5^{zko1049a/zko1049a}; sd2Tg/sd2Tg
Observed In:	erythrocyte differentiation nucleate erythrocyte nucleate erythrocyte mitochondrial crista nucleate erythrocyte mitochondrion
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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