FIGURE

Figure 6

ID

ZDB-FIG-211111-57

Publication

Ghanta et al., 2021 - 5' modifications improve potency and efficacy of DNA donors for precision genome editing

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Figure 6

5?-Modifications suppress donor ligation reactions.

(A) Model for mechanisms of concatemer formation for unmodified donors is shown. (B) Southern blot of unmodified and triethylene glycol (TEG)-modified double-stranded DNA (dsDNA) (566 bp) nucleofected into HEK293T cells and collected at indicated time points. Concatemerization of unmodified DNA is visualized as ladders; 566 bp DNA and 13 kb long DNA are used as size markers (m). Number of GUIDE-seq reads with unmodified and TEG-modified short dsDNA (34 bp) integration for (C) whole genome and (D) on-target (ARHGEF9) and six previously validated off-target loci are plotted. Data from two biological replicates is shown.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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