Fig. 1

Schematic diagram illustrating the separation of GFP-expressing and non-GFP-expressing cells from stress-treated zebrafish embryos of transgenic line huORFZ using LMD methods followed by microarrays. Graphical representation of two microarray expression analyses. A total of 298 over-represented transcripts and 104 under-represented transcripts were obtained from both microarrays. Upper panels: top view of EGFP expressed in zebrafish embryos derived from zebrafish transgenic line huORFZ under non-stress and heat-shock treatment. Middle panels: lateral view of endouc expression patterns in embryos under non-stress condition and treated with heat-shock. Lower panels: top view of endouc expression patterns. Quantitative RT–PCR determined the relative expression level of endouc mRNA in non-stress- and heat-shock-treated embryos. Values representing the non-stress- and heat-shocked conditions were calculated from three independent experiments and presented as mean ± SD (n = 3). Student's t-test was used to determine significant differences between each group (***P < 0.001). The endouc and ela3l (negative control) mRNAs were separately microinjected into zebrafish embryos from huORFZ at one-cell stage. Upper panels: observation of GFP signal expressed in huORFZ embryos at 72 hpf. Lower panels: magnification of pictures corresponding to the upper panels.