Figure 4
- ID
- ZDB-FIG-211029-119
- Publication
- Félix et al., 2021 - Cartilage Acidic Protein a Novel Therapeutic Factor to Improve Skin Damage Repair?
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Transcript level of 15 genes quantified by RT-qPCR in SJD.1 fibroblast cells exposed to three control and CRTAC1 proteins (hCRTAC1-A, dlcrtac1a and dlcrtac1b). In intact cells (control) no damage was inflicted and other samples were collected immediately after the scratch (AS_0h) or at 6 h (AS_6h) and 24 h (AS_24h) after the scratch. Data corresponded to the mean ± SEM of four independent experiments for the control and dlCrtac1a and three independent experiments for hCRTAC1-A and dlCrtac1b. Gene expression levels (2???CT) were normalized in relation to intact cells using the geometric mean of two reference genes (18S and ef1?) and the data were analyzed using a two-way ANOVA followed by Tukey?s Multiple Comparison test. The statistical analysis was performed using GraphPad Prism version 7.0a. p < 0.05 (*) and p < 0.01 (**) were considered significant. Gene symbols are indicated, for the full gene name consult Table 1. |